Enantiomeric separation of amino acids and nonprotein amino acids using a particle-loaded monolithic column

2000 ◽  
Vol 21 (15) ◽  
pp. 3145-3151 ◽  
Author(s):  
Masaru Kato ◽  
Maria T. Dulay ◽  
Bryson Bennett ◽  
Jing-ran Chen ◽  
Richard N. Zare
Keyword(s):  
Amino Acids ◽  
Monolithic Column ◽  
Enantiomeric Separation ◽  
Nonprotein Amino Acids

scholarly journals Monolithic Silica Capillary Columns with Improved Retention and Selectivity for Amino Acids

Separations ◽  
2018 ◽  
Vol 5 (4) ◽  
pp. 48 ◽  
Author(s):  
Dana Moravcová ◽  
Josef Planeta
Keyword(s):  
Amino Acids ◽  
Ammonium Acetate ◽  
High Selectivity ◽  
Monolithic Column ◽  
Ammonium Hydroxide ◽  
Capillary Columns ◽  
Silica Monolith ◽  
Monolithic Capillary Columns ◽  
Modification Procedure ◽  
Silica Capillary

A strategy for the preparation of silica-based monolithic capillary columns (150 × 0.1 mm) with high selectivity to amino acids is presented. The zwitterionic columns were prepared by coating the silica monolith with [2-(methacryloyloxy)ethyl]-dimethyl-(3-sulfopropyl)-ammonium hydroxide via 3-(trimethoxysilyl)propyl methacrylate. The columns were evaluated under isocratic conditions in hydrophilic interaction liquid chromatography. The best separation of amino acids was obtained on the monolithic column prepared by a stepwise modification procedure where the modification step was repeated four times. The mixture of fifteen amino acids was separated within 13 min using the mobile phase consisting of 75% acetonitrile and 25% 5 mmol/L ammonium acetate at pH 4.5.

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